The dataset provides information on soil chemistry from a 10 year chronosequence sample of restoration in southern Australia. The parameters include: A) Physical properties- Soil moisture (%), Gravel (%) - ( >2.0 mm), Soil Texture, i.e.Course Sand (%) (200-2000 µm), Fine Sand (%) - (20-200 µm), Sand (%), Silt (%) (2-20 µm), Clay (%) (<2 µm), and B) Chemical properties- such as, Ammonium Nitrogen (mg/Kg), Nitrate Nitrogen (mg/Kg), Phosphorus Colwell (mg/Kg), Potassium Colwell (mg/Kg), Sulphur (mg/Kg), Organic Carbon (%), Conductivity (dS/m), pH (CaCl2), pH (H2O), DTPA Copper (mg/Kg), DTPA Iron (mg/Kg), DTPA Manganese (mg/Kg), DTPA Zinc (mg/Kg), Exc. Aluminium (meq/100g), Exc. Calcium (meq/100g), Exc. Magnesium (meq/100g), Exc. Potassium (meq/100g), Exc. Sodium (meq/100g) and Boron Hot CaCl2 (mg/Kg). This data would have application for land managers. The soil chemistry data is also related to the eDNA OTU table published on "https://doi.org/10.4227/05/5878480a91885", titled "Revegetation rewilds the soil bacterial microbiome of an old field. Part 1: OTU raw data matrix", and as such it would have an appeal to researchers undertaking a meta-analysis on eDNA and restoration outcomes.

This data contains soil physico-chemical characteristics collected at the Daintree Rainforest, Cape Tribulation site between 2007 - 2015.

Soil collection and analysis of chemical and physical attributes was carried out at the Great Western Woodlands site to provide contextual data for the Biomes of Australian Soil Environments (BASE) soil microbial diversity project.

This data contains soil physico-chemical characteristics collected at the Great Western Woodlands site in 2012 and 2014.

This data contains soil physico-chemical characteristics collected at the Cumberland Plain site in 2013.

This data contains soil physico-chemical characteristics collected at the Whroo Dry Eucalypt site in 2015.

Experimental sites were established in the northern wheat-growing district of western Australia (Lat -29.66°, Long 116.18°) in August 2017, and monitored through to November 2019. We selected five planted old field sites with similar soil types and vegetation composition. Old fields were planted with York gum (Eucalyptus loxophleba Benth.) and dominant shrubs as understorey. At the time of sampling in 2017, vegetation age ranged from 8–13 years and distance from remnant measured 279 m (± 162 m). We established two control and two treatment plots, each measuring 5 m x 5 m, in the interrows of five planted old field sites. Both treatments were randomly assigned to plots within each site. Between August and early November 2017, we measured a total of 30 response variables at each of the control and treatment plots. Response variables included soil physical and chemical properties (bulk density, penetration resistance, soil moisture, nitrogen and carbon pools), microbial biomass, decomposition rate of roiboos and green tea as per the standardized Tea Bag Index (TBI) protocol, herbaceous vegetation cover and richness, and ant abundance and richness, as well as abundance and richness of ant functional groups.

The dataset accompanies the paper by Zemunik et al. (2016), which used the Jurien Bay dune chronosequence to investigate the changes in the plant community diversity and turnover in response to long-term soil development. The Jurien Bay chronosequence is located in the Southwest Australian biodiversity hotspot, in an area with an extremely rich regional flora. The dataset consists of both flora and soil data that allows all analyses presented in the paper (Zemunik et al. 2016) to be independently investigated. The dataset is an update to that previously supplied for a prior study (Zemunik et al. 2015; DOI 10.4227/05/551A3DDE8BAF8). The study used a randomised stratified design, stratifying the dune system of the chronosequence into six stages, the first three spanning the Holocene (to ~6.5 ka) and oldest spanning soil development from the Early to Middle Pleistocene (to ~2 Ma). Floristic surveys were conducted in 60 permanent 10 m × 10 m plots (10 plots in each of six chronosequence stages). Each plot was surveyed at least once between August 2011 and March 2012, and September 2012. To estimate canopy cover and number of individuals for each plant species within the 10 m × 10 m plots, seven randomly-located 2 m × 2 m subplots were surveyed within each plot. Within each subplot, all vascular plant species were identified, the corresponding number of individuals was counted and the vertically projected vegetation canopy cover was estimated. Surface (0-20 cm) soil from each of the 420 subplots was collected, air dried and analysed at the Smithsonian Tropical Research Institute in Panama, for a range of chemical and physical properties: total and resin soil phosphorus; total nitrogen and dissolved organic nitrogen; soil total and organic carbon; exchangeable calcium (Ca), iron (Fe), potassium (K), magnesium (Mg), manganese (Mn) and sodium (Na); Mehlich-III extractable iron, magnesium, copper (Cu) and zinc (Zn); and pH (measured in H20 and CaCl2). Nutrient-acquisition strategies were determined from the literature, where known, and from mycorrhizal analyses of root samples from species with poorly known strategies. Most of the currently known nutrient-acqusition strategies were found in the species of the chronosequence. Previous studies in the Jurien Bay chronosequence have established that its soil development conforms to models of long-term soil development first presented by Walker and Syers (1976); the youngest soils are N-limiting and the oldest are P-limiting (Laliberté et al. 2012). However, filtering of the regional flora by high soil pH on the youngest soils has the strongest effect on local plant species diversity (Laliberté et al. 2014). The update involved modification to species names due to taxonomic changes and the inclusion of additional soil analyses, not present in Zemunik et al. (2015). The additional soil variables (additional to DOI 10.4227/05/551A3DDE8BAF8) were exchangeable Ca, K, Al, Mg, Mn and Na, measured for all 420 subplots; and Cu, Fe, Mn and Zn, extracted in Mehlich III solution, for each of the 60 plots. References Laliberté, E., Turner, B.L., Costes, T., Pearse, S.J., Wyrwoll, K.H., Zemunik, G. & Lambers, H. (2012) Experimental assessment of nutrient limitation along a 2-million-year dune chronosequence in the south-western Australia biodiversity hotspot. Journal of Ecology, 100, 631-642. Walker, T.W. & Syers, J.K. (1976) The fate of phosphorus during pedogenesis. Geoderma, 15, 1-19. Zemunik, G., Turner, B.L., Lambers, H. & Laliberté, E. (2015) Diversity of plant nutrient-acquisition strategies increases during long-term ecosystem development. Nature Plants 1, Article number: 15050, 1-4. Zemunik, G., Turner, B.L., Lambers, H. & Laliberté, E. (2016) Increasing plant species diversity and extreme species turnover accompany declining soil fertility along a long-term chronosequence in a biodiversity hotspot. Journal of Ecology.

Soil collection and analysis of chemical and physical attributes was carried out at the Daintree Rainforest, Cape Tribulation site to provide contextual data for the Biomes of Australian Soil Environments (BASE) soil microbial diversity project.

This data contains soil physico-chemical characteristics collected at the Daintree Rainforest, Cow Bay site between 2011 - 2014.